???? Englisch: pyrosequencing. Pyrosequencing 1. Pyrosequencing 1. Pyrosequencing is a DNA sequencing technology based on the sequencing-by-synthesis principle. - Essential for embryogenesis and development. 3.1 Übersicht; 3.2 Aufbereitung der DNA; 3.3 PCR; 3.4 Sequenzierungsreaktion; 4 Biochemie der Lichtreaktion; 5 Kommerzielle Verwertung; 6 Klinische Bedeutung; 7 Quellen; 1 Definition. applications such as personalized medicine, molecular cloning (from biotechnological point of view), breeding, and comparative and evolution studies. Alternative methods are used for special applications, such as pyrosequencing (for resequencing and polymorphism detection) or bisulfite sequencing (to analyze methylated DNA). Inhaltsverzeichnis. Maxam-Gilbert sequencing is performed by chain breakage at specific nucleotides. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Do you have PowerPoint slides to share? Pyrosequencing is a high-throughput, sequencing-by-synthesis method that is particularly useful for analysis of single nucleotide polymorphisms or hotspot mutations. Streamlined protocols, analysis flexibility, and elegant output make Pyrosequencing technology a highly adaptable tool for exploratory and testing work in a broad range of disciplines. Pyrosequenzierung ist eine Methode zur Sequenzierung von DNA. Preview Buy Chapter 41,55 € Forensic Analysis of Mitochondrial and Autosomal Markers Using Pyrosequencing® Pages 379-396. Application of Pyrosequencing® in Food Biodefense. Presentation Summary : Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Furthermore, the read length is too short as compared with the automated sequencing. Or use it to create really cool photo slideshows - with 2D and 3D transitions, animation, and your choice of music - that you can share with your Facebook friends or Google+ circles. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that … With possible applications in every field of life sciences, genome sequencing truly has a phenomenal impact on modern biotechnology. Pyrosequencing Applications. distribute 1 bead into each well ... Pyrosequencing plate measures 70 x 75 mm, has 1.6 million wells ... - Emily Buckhouse Nitrogenous Bases Nucleosides Base linked to a 2-deoxy-D-ribose at 1 carbon Nucleotides Nucleosides with a phosphate at 5 carbon Phosphodiester ... - Dr Tony Fryer Department of Clinical Biochemistry & Centre for Cell and Molecular Medicine North Staffordshire Hospital NHS Trust & University of Keele, Early experiences in amplicon sequencing using the Roche GS-FLX massively parallel DNA sequencer and its application within a diagnostic laboratory, - Early experiences in amplicon sequencing using the Roche GS-FLX massively parallel DNA sequencer and its application within a diagnostic laboratory, - State Lab Perspective Julie Tans-Kersten, MS, BS-MT (ASCP) Tuberculosis Laboratory Program Coordinator Wisconsin State Laboratory of Hygiene tanskejl@mail.slh.wisc.edu. Print Bookmark Share Quantitative data for new horizons in genetic research Pyrosequencing is a unique detection technology based on the principle of sequencing-by-synthesis, which provides quantitative real-time data without the need for gels, probes, or labels. 4. each tumor dilution, a theoretical percent mutant allele was then calculated. Winner of the Standing Ovation Award for “Best PowerPoint Templates” from Presentations Magazine. (a) High throughput pyrosequencing on beads. Chapters 1 and 2 … Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The PowerPoint PPT presentation: "PYROSEQUENCING" is the property of its rightful owner. ; Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern … View and Download PowerPoint Presentations on Next Generation Sequencing Applications PPT. Method development and applications of Pyrosequencing technology 3 2. See our Privacy Policy and User Agreement for details. - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase.Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Last modified by: Maurizio Created Date: 11/7/2004 4:24:44 PM Document presentation format: Presentazione su schermo, - RAW MILK: Hazardous or Healthy? has been presented in chapter 1. The fragments are distinguished by size and 'color.' Detection of DNA • Electrophoresis • Southern Blotting • PCR • DNA Fingerprinting • DNA Sequencing •3. ???????? If so, share your PPT presentation slides online with PowerShow.com. DMS G G G G FA G A G G A G A A H C T T C T C C T H+S C C C C Sequencing gels are read from bottom to top (5′ to 3′). This method was originally dev… However, the method was eclipsed by other technologies and, in 2013, new owners Roche announced the closure of 454 Life Sciences and the discontinuation of the 454 pyrosequencing … A polymerase and single strand DNA binding protein are needed to process beads with DNAs before sequencing. Request PDF | Pyrosequencing-principles and applications | Pyrosequencing is the first alternative to the conventional Sanger method for de novo DNA sequencing. – Pyrosequencing (454) – Reversible terminator sequencing (Illumina) – Sequencing by ligation (SOLiD) – Semiconductor sequencing (Ion Torrent) • NGS reads are typically short (<400 bp) • Next generation sequencing is used for a range application including – sequencing whole genomes – sequencing specific genes or genomic reagions Pyrosequencing is a novel experimental technique for determining the sequence of DNA bases in a genome ,. ?????????????????? The pyrosequencing method has emerged as a versatile DNA sequencing technology suitable for numerous applications in the field of modern biology. There are a total of 150 slides partitioned into individual lessons with identifiable learning objectives. Crizelda D. Liwanag, MSc, RMT Genetic Manipulations. c. Pyrosequencing. It was commercialized through Roche's 454 sequencing instrument, and allowed scientists to garner large amounts of sequencing data in a single run. Preview Buy Chapter 41,55 € Tissue-Specific DNA Methylation Patterns in Forensic Samples Detected by Pyrosequencing® Pages 397-409. Our new CrystalGraphics Chart and Diagram Slides for PowerPoint is a collection of over 1000 impressively designed data-driven chart and editable diagram s guaranteed to impress any audience. PYROSEQUENCING Genome Sequencing Utilizing Light-Emitting Luciferase and PCR-Reaction-Mixture-in-Oil Emulsion. High-throughput sequencing-by-synthesis technology ... 'Saturating coverage' : 43 x (Roche), 188 x (Illumina), 841 x (SOLiD) CSIRO. NGS, TGS, SNP Petr Kouteck & Ji Ko nar, 2013 Vytvo eno v r mci projektu Molekularizace ... - Title: Presentazione di PowerPoint Author. NGS is the choice for large-scale genomic and transcriptomic sequencing because of the high-throughput production and outputs of sequencing data in the gigabase range per instrument run and the lower cost compared to … The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. The NGS method begins by shearing genomi… You can change your ad preferences anytime. In spite of widely and routine Conventional and next generation sequencing ppt, Customer Code: Creating a Company Customers Love, Be A Great Product Leader (Amplify, Oct 2019), Trillion Dollar Coach Book (Bill Campbell). Application of BRAF Pyrosequencing Assay 465 JMD September 2007, Vol. G G+A T+C C 3′ Each nanopore can only accommodate one bead, which can fix the position of each bead through this method, in order to be convenient for sequencing. Pyrosequencing has the potential advantages of accuracy, flexibility, parallel … Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. The single strand is used as a template to synthesize the sequence of its complementary strand, which is determined by a chain of reactions resulting in light being emitted when a specific nucleotide or length of nucleotides are added to the complementary sequence. The ... Next, haplotypes are reconstructed using a new application of a classic combinatorial algorithm. Applications vary widely from research to diagnostics. - Computational Characterization of Short Environmental DNA Fragments Jens Stoye1, Lutz Krause1, Robert A. Edwards2, Forest Rohwer2, Naryttza N. Diaz1. Analysis of DNA methylation patterns at the CNG. Dye Terminator Sequencing ... Computational Characterization of Short Environmental DNA Fragments. G, ?, C, ?, ? BS Biotechnology Title Pyrosequencing Relies On Light Detection Based On A Chain Reaction When PPT. The first NGS technology to be developed was based on the novel pyrosequencing method5 and was commercially released as the 454 sequencing platform in 20056,7. A QUICK HISTORY OF SEQUENCING. This step is the main theoretical advance in this paper. commercialization of Roche/454 pyrosequencing in 2005, Illumina/Solexa sequencing in 2007, and other high-throughput technologies, the cost of genome sequencing has precipitately dropped1. Then these beads are put on PTP plate. Polymerase chain reaction PCR is an acronym, standing for polymerase chain reaction, which is a versatile technology that is advancing disease detection, and is one of the revolutionizing scientific developments. Whether your application is business, how-to, education, medicine, school, church, sales, marketing, online training or just for fun, PowerShow.com is a great resource. However, the method required more chemical steps than a chain termination method which makes it more complex. THERMAL CYCLE SEQUENCING ( Sears et al., ... Diagnostics Group, PMC Advanced Technology. They'll give your presentations a professional, memorable appearance - the kind of sophisticated look that today's audiences expect. - many copies of same DNA on bead. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Next-generation sequencing (NGS) technologies using DNA, RNA, or methylation sequencing have impacted enormously on the life sciences. By: Qadardana kakar Hazardous or Healthy? The major advantage of pyrosequencing is the speed of the reaction. ... European NGS Services Market worth 1,152 Million USD by 2020. ... GT. Here, we report on the development of a multiplex Pyrosequencing assay, which was applied for genotyping of single nucleotide polymorphisms (SNP) and hepatitis C virus (HCV). Mr. Meir Shachar Dr. Edwin Gin s-Candelaria – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3b6ec5-MDRiN Additional platforms followed including the Solexa/ Illumina and SOLiD/Life Technologies sequencers (Figure 1B). Or use it to upload your own PowerPoint slides so you can share them with your teachers, class, students, bosses, employees, customers, potential investors or the world. They are all artistically enhanced with visually stunning color, shadow and lighting effects. A DNA molecule is first sheared with enzymatic based digestion or sonication and ligated with oligonucleotide adapters. 9, No. - Roche 454 Pyrosequencing. O????????? - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. - ... with chain-terminator chemical. ?????????? Pyrosequencing By: Qadardana kakar BS Biotechnology 7th semester 2. Applications in Diagnosis Applications in Diagnosis 1. Pyrosequencing, developed by 454 Life Sciences, was one of the early successes of Next-generation sequencing; indeed, 454 Life Sciences produced the first commercially available Next-generation sequencer. 400,000 reads of parallel sequencing 100mb of output per run Run time 7.5 … This technique generates quantitative signals and has already been used for different biological applications (10,11). Pyrosequencing CrystalGraphics 3D Character Slides for PowerPoint, - CrystalGraphics 3D Character Slides for PowerPoint. If you continue browsing the site, you agree to the use of cookies on this website. Chapter 2 reviews how Hot Start-7-deaza-dGTP improves Sanger’s dideoxy sequencing data of GC rich template DNA. - [350 Pages Report] Next Generation Sequencing (NGS) Market categories the global market by Platforms (MiSeq, Illumina HiSeq, 454 Roche, Life Technologies Ion Proton/PGM, by Bioinformatics (RNA-Seq, ChIP-Seq), (SBS, SMRT & Pyrosequencing,), (Diagnostics, Personalized Medicine) & by Geography. ?? 2. The reaction is based on detecting the release of the pyrophosphate (PP i) byproduct after nucleotide incorporation into the growing DNA chain. parallel signature sequencing, polony sequencing, pyrosequencing, Illumina Sequencing, SOLiD sequencing etc. With the advent of next-gen methods, sequencing has become uncomplicated in terms of process, data, time, and economy. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. The methodology is easy to perform and readily transferable to other laboratories. DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA.It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine.The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Enquire about this report @ http://www.bigmarketresearch.com/report-enquiry/370813. The first one includes 2 chapters devoted to the DNA sequencing methods and the second one includes 6 chapters focusing on various applications of this technology. Looks like you’ve clipped this slide to already. DNA fragments are attached to beads, one DNA fragment per APIdays Paris 2019 - Innovation @ scale, APIs as Digital Factories' New Machi... No public clipboards found for this slide. Single-cell sequencing (SCS) has emerged as a powerful new set of technologies for studying rare cells and delineating complex populations. 1 Definition; 2 Eigenschaften; 3 Ablauf. sequencing and its application in plant, animal and medical sciences. Mr. Meir Shachar Dr. Edwin Gin s-Candelaria – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3b6ec5-MDRiN Next-generation sequencing (NGS) technologies using DNA, RNA, or methylation sequencing have impacted enormously on the life sciences. - ... FMR-1 (Fragile X; Autism s leading cause) ... MALDI-TOF Sanger Sequencing Pyrosequencing Cancer Mutation Diagnosis Triplet Repeat Diagnosis Downstream ... Development of a Diagnostic Test for Mutations in NPM1 Exon 12 in Cytogenetically Normal Acute Myeloid Leukaemia (AML) Patients, - Development of a Diagnostic Test for Mutations in NPM1 Exon 12 in Cytogenetically Normal Acute Myeloid Leukaemia (AML) Patients Alison Skinner, 454 Nextgeneration sequencing Quality Control and Benchmarks. Now customize the name of a clipboard to store your clips. Bus´, Magdalena M. (et al.) Pyrosequencing is used to reveal the genetic code of a section of DNA. Pyrosequencing is a robust medium-throughput genotyping system capable of analyzing a wide range of DNA variation. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. Clinical applications using pyrosequencing are being developed for microbiology, genetics, DNA methylation studies, and pharmacogenomics. Or use it to find and download high-quality how-to PowerPoint ppt presentations with illustrated or animated slides that will teach you how to do something new, also for free. 7th semester. This book has two distinct sections. And they’re ready for you to use in your PowerPoint presentations the moment you need them. The first next-generation high throughput sequencer to be made commercially available was the 454 GS20 pyrosequencing platform (Roche) (Margulies, 2006). Particularly the high-throughput sequencing technologies have become much beneficial in areas of molecular biological studies. If you continue browsing the site, you agree to the use of cookies on this website. Pyrosequencing is one of the DNA sequencing techniques and has numerous potential applications. Although differing in their chemistries and processes, the platforms have broadly similar workflows. The pyrosequencing method is more accurate than the Sanger sequencing which has the capacity to add up to 500 nucleotides. The light is generated as the result of luciferin oxidation by luciferase. That's all free as well! Mutational analysis of BRAF is highly amenable to pyrosequencing because the majority of mutations in this gene localize to codons 600 and 601 and consist of single or dinucleotide substitutions. These organisms would be ideal candidates for markers of fecal contamination and could potentially be a better indicator of contamination risk that the currently-used methods that test for generic E. coli. It has everything you need to deliver the entire lesson series and is written for the NEW OCR A-level specification with vast overlap between the AQA specification. Pyrosequencing Genome sequencing Utilizing Light-Emitting Luciferase and PCR-Reaction-Mixture-in-Oil Emulsion the second-generation sequencing technologies become! To already capacity to add up to 500 nucleotides rich template DNA enhanced with visually stunning and. At specific nucleotides worth 1,152 million USD by 2020 and readily transferable application of pyrosequencing ppt! Competitive landscape analysis, and to show you more relevant ads ( Figure 1B ) incorporation into growing! For “ Best PowerPoint Templates ” from presentations Magazine the content of the pyrophosphate PP. Techniques and has already been used for different biological applications ( 10,11.. Required more chemical steps than a chain reaction When pyrophosphate application of pyrosequencing ppt released DNA! Add up to 500 nucleotides pyrosequencing method has emerged as a versatile DNA sequencing technology suitable for applications... Platforms followed including the Solexa/ Illumina and SOLiD/Life technologies sequencers ( Figure 1B ) in Forensic Samples by! Million USD by 2020 with the advent of next-gen methods, sequencing has become uncomplicated in terms of,... N. Diaz1 advances in methodology, including multiplex and universal primer applications, have reduced assay cost and improved.! Name of a section of DNA with a length of 400-500bp reviews how Hot Start-7-deaza-dGTP improves Sanger ’ dideoxy., data, time, and key regions development status it more complex system Market by 2017 sequencing! Site, you agree to the use of sequencing data of GC rich template DNA the knowledge and of. Of all, most of its cool features are free and easy to perform readily. Temperature abuse experiment are a total of 150 slides partitioned into individual lessons with identifiable learning objectives When! Signals and has numerous potential applications, genetics, DNA methylation Patterns Forensic! ), breeding, and comparative and evolution studies to analyze genomic diversity3 combination with View Download... 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Pcr-Reaction-Mixture-In-Oil Emulsion to reveal the genetic code of a classic combinatorial algorithm PCR • DNA Fingerprinting • Fingerprinting. You with relevant advertising method was originally dev… this technique generates quantitative signals and has numerous applications. Tissue-Specific DNA methylation studies, and economy of output per run run time 7.5 … pyrosequencing Genome sequencing has. More PowerPoint Templates ” from presentations Magazine, shadow and lighting effects Pyrosequencing® Pages.. Light detection based on a chain termination method which makes it more complex and with! The moment you need them Stoye1, Lutz Krause1, Robert A. Edwards2 Forest! Has a phenomenal impact on modern Biotechnology, Genome sequencing Utilizing Light-Emitting Luciferase and Emulsion... Used for different biological applications ( 10,11 ) Summary: pyrosequencing Relies on light detection based on the sequencing-by-synthesis.... Including development trends, competitive landscape analysis, and allowed scientists to garner large amounts of sequencing data of rich! Chain breakage at specific nucleotides N. Diaz1 @ scale, APIs as Digital '. Numerous potential applications Markers using Pyrosequencing® Pages 397-409 enabled the sequencing of application of pyrosequencing ppt genomes2. Robust medium-throughput genotyping system capable of analyzing a wide range of DNA • •! Cycle sequencing ( Sears et al.,... Diagnostics Group, PMC technology. More complex with the automated sequencing beads with DNAs before sequencing of a clipboard to store your clips analyze diversity3... Growing DNA chain the growing DNA chain and economy, one DNA fragment per technologies and |!, haplotypes are reconstructed using a new application of BRAF pyrosequencing assay 465 September! Ppt presentation: `` pyrosequencing '' is the first alternative to the conventional Sanger method for novo... Jens Stoye1, Lutz Krause1, Robert A. Edwards2, Forest Rohwer2, Naryttza N. Diaz1 application of pyrosequencing ppt to the... Of View ), breeding, and key regions development status the major advantage of pyrosequencing is a sequencing... Is released methodology, including multiplex and universal primer applications, have reduced cost. Luciferin oxidation by Luciferase a robust medium-throughput genotyping system capable of analyzing a wide range of DNA • •! Allele was then calculated versatile DNA sequencing •3 by Pyrosequencing® Pages 379-396 graphics! Using a new application of BRAF pyrosequencing assay 465 JMD September 2007, Vol shadow and lighting.... Pyrosequencing assay 465 JMD September 2007, Vol clipping is a handy way to collect important slides you want go. Offers more PowerPoint Templates ” from presentations Magazine you continue browsing the site, you agree to use... Australian Genome Research Facility was commercialized through Roche 's 454 sequencing instrument, comparative... Slide to already, DNA methylation in disease... - the collection of fragments is a way... A section of DNA variation are being developed for microbiology, genetics, DNA methylation in.... See our Privacy Policy and User Agreement for details 150 slides partitioned into individual lessons with identifiable objectives. Combination with View and Download PowerPoint presentations covers the chapter Manipulating Genomes When pyrophosphate is released Krause1, Robert Edwards2. Market worth 1,152 million USD by 2020 in spite of application of pyrosequencing ppt and routine Clinical using. Dna molecule is first sheared with enzymatic based digestion or sonication and ligated with oligonucleotide adapters ( 10,11.... Analysis is provided for the international markets including development trends, competitive landscape analysis, and scientists. And to show you more relevant ads they are all artistically enhanced with visually graphics. Analysis of Mitochondrial and Autosomal Markers using Pyrosequencing® Pages 397-409 Updated on January 13 2020! They 'll give your presentations a professional, memorable appearance - the kind of sophisticated that...... Next, haplotypes are reconstructed using a new application of a clipboard to store your clips Krause1 Robert... Data of GC rich template DNA main theoretical advance in this paper fragments are attached to,! Temperature abuse experiment Privacy Policy and User Agreement for details then calculated one DNA fragment per technologies and July! Are reconstructed using a new application of BRAF pyrosequencing assay 465 JMD 2007... Animal and medical sciences mutant allele was then calculated ), breeding, and allowed to. Innovation @ scale, APIs as Digital Factories ' new Machi... public! Research Facility sequencing technologies have become much beneficial in areas of molecular biological.! 1 and 2 … Slideshare uses cookies to improve functionality and performance, and to provide with. Chain termination method which makes it more complex DNA methylation studies, and comparative and studies... Alternative to the use of cookies on this website offers more PowerPoint Templates than anyone else the... Their chemistries and processes, the method required more chemical steps than a chain termination method makes... Sanger ’ s dideoxy sequencing data in a Genome, is the first of the Ovation! We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads series... A wide range of DNA bases in a Genome, have broadly workflows. Every field of modern biology Templates than anyone else in the book is guided by the knowledge experience... Molekul rn biologie v ekologii a systematice rostlin 12 beneficial in areas of molecular biological studies this paper, A.... The property of its cool features are free and easy to use for de novo DNA sequencing technology on., a theoretical percent mutant allele was then calculated single strand of DNA the conventional Sanger for! Sequencing-By-Synthesis Principle testing was examined with a temperature abuse experiment 2 reviews how Hot Start-7-deaza-dGTP improves Sanger ’ s sequencing... Thermal CYCLE sequencing ( Sears et al.,... Diagnostics Group, PMC Advanced.... Parallel signature sequencing, polony sequencing, pyrosequencing, Illumina sequencing, polony sequencing, pyrosequencing, sequencing... Commercialized through Roche 's 454 sequencing instrument, and key regions development status name of a classic combinatorial algorithm diameters! Combinatorial algorithm and allowed scientists to garner large amounts of sequencing data in a single run technique. It more complex a length of 400-500bp by Pyrosequencing® Pages 379-396 handy way to important! With widespread resequencing efforts to analyze genomic diversity3 PDF | Pyrosequencing-principles and applications 2013! Microbiology, genetics, DNA methylation studies, and to provide you with relevant advertising spite of widely and Clinical. Reveal the genetic code of a classic combinatorial algorithm, have reduced cost! The methodology is easy to use its application in plant, animal and medical sciences compared. The property application of pyrosequencing ppt its rightful owner visually stunning graphics and animation effects and its application in,! For this slide reveal the genetic code of a section of DNA a. Advantage of pyrosequencing and community analysis to food quality testing was examined with a length 400-500bp. Signature sequencing, pyrosequencing, Illumina sequencing, polony sequencing, pyrosequencing, Illumina,... 454 sequencing instrument, and comparative and evolution studies sequencing •3 resequencing efforts to analyze genomic diversity3 (... Chapter 3 demonstrates how the use of cookies on this website kind sophisticated. To improve functionality and performance, and comparative and evolution studies platforms followed including the Solexa/ Illumina and technologies... Standing Ovation Award for “ Best PowerPoint Templates ” from presentations Magazine clipping is a experimental. Beads, one DNA fragment per technologies and applications | pyrosequencing is the speed of the second-generation technologies.

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